Vincristine and ɛ-viniferine-loaded PLGA-b-PEG nanoparticles: pharmaceutical characteristics, cellular uptake and cytotoxicity.
- Resource Type
- Article
- Authors
- Öğünç, Yüksel; Demirel, Müzeyyen; Yakar, Arzu; İncesu, Zerrin
- Source
- Journal of Microencapsulation. Feb2017, Vol. 34 Issue 1, p38-46. 9p.
- Subject
- *NANOPARTICLES
*CELL-mediated cytotoxicity
*CANCER treatment
*CANCER research
*THERAPEUTICS
TUMOR growth prevention
- Language
- ISSN
- 0265-2048
The objective of this study was to prepare the ɛ-viniferine and vincristine-loaded PLGA-b-PEG nanoparticle and to investigate advantages of these formulations on the cytotoxicity of HepG2 cells. Prepared nanoparticle has shown a homogeneous distribution with 113 ± 0.43 nm particle size and 0.323 ± 0.01 polydispersity index. Zeta potential was determined as −35.03 ± 1.0 mV. The drug-loading percentages were 6.01 ± 0.23 and 2.01 ± 0.07 for ɛ-viniferine and vincristine, respectively. The cellular uptake efficiency of coumarin-6-loaded nanoparticles was increased up to 87.8% after 4 h. Nanoparticles loaded with high concentrations of both drugs showed a cytotoxic effect on HepG2 cells, having the percentage of cell viability of between 43.23% and 47.37%. Unfortunately, the percentage of apoptotic cells after treated with drugs-loaded nanaoparticles (10.93%) was similar to free forms of drugs (12.1%) that might be due to low ɛ-viniferine release in biological pH at 24 h. [ABSTRACT FROM PUBLISHER]