Here, we report multiwalled carbon nanotubes (MWCNTs) functionalized with γ‐cyclodextrins (γCD) as a novel electrochemical strategy for Rutin determination, showing superior performance than β‐cyclodextrins (βCD) modified MWCNTs, suggesting an adequate environment for host‐guest interactions. Under optimized conditions, the sensor showed a linear range of 39–975 nmol L−1 and a limit of detection of 7 nmol L−1. When tested with quercetin, catechin, and caffeine, the platform presented high selectivity with an interference response <10 %. The method was employed to quantify Rutin in spiked pharmaceutical and herbal extracts, providing recovery of 93–98.4 %. Also, HPLC‐PDA confirmed the method's accuracy. [ABSTRACT FROM AUTHOR]