The fate of nanoparticles in natural waters is affected by the combination of various factors, especially the flow disturbance which plays a decisive role in the transport of nanoparticles. This study investigated the aggregation behavior of CeO 2 nanoparticles (NPs) in natural waters by using a unique instrument to simulate flow disturbance. The results indicated that, in the absence of a shear force, the CeO 2 NPs formed linear, chain-like aggregates in seawater, owing to the high IS, which compressed the electrical double layer of particles. On the other hand, the NPs formed more compact aggregates in lake water, owing to an ion bridge effect between the NPs and the dissolved organic matter (DOM). It was also found that shear forces affected the aggregation behavior of the NPs. A low shear force promoted the aggregation of the NPs by increasing the collision efficiency while the aggregates were broken by a high shear force. Remarkably, the NPs maintained their potential for continuous aggregation when the slow stirring was reintroduced, suggesting that the aggregates began to grow again under renewed stirring. The results of this study could help in predicting the fate and transport behavior of CeO 2 NPs in actual aquatic environments. [ABSTRACT FROM AUTHOR]