ACETYLCHOLINESTERASE AND CHOLINE UPTAKE IN STRIATUM FROM RATS WITH VARYING SLEEPING TIMES.
- Resource Type
- Article
- Authors
- CHUMAKOVA, OLGA V.; LIOPO, ANTON V.
- Source
- Alcohol & Alcoholism. Mar1996, Vol. 31 Issue 2, p217-220. 4p.
- Subject
- *ACETYLCHOLINESTERASE
*CHOLINE
*LABORATORY rats
*SLEEP
*ETHANOL
*INJECTIONS
- Language
- ISSN
- 0735-0414
Male rats were injected with ethanol as a 25% solution (3.5 g/kg body weight) and then the duration of the retention of the righting reflex (DRR) as well as the duration of ethanol-induced sleep (DEIS) were recorded. On the basis of DEIS in 60 rats. we selected four groups of six rats each: short-sleep group (SS. 34 ± 5 min). intermediate-sleep (IS. 118 ±3 min). long-sleep (LS. 186 ±5 min) and non-sleep rats (NS. 0 min). The striatal crude mitochondrial fraction was assayed for acetylcholinesterase activity (AChE, EC 3.1.1.7) and high-affinity choline uptake. NS rats manifested similarity of the above parameters to those determined in SS and IS rats. LS rats were distinguished by a higher AChE activity in comparison with NS animals. [ABSTRACT FROM PUBLISHER]