Thestability and longevity of surface-stabilized lubricant layersis a critical question in their application as low- and nonfoulingslippery surface treatments in both industry and medicine. Here, weinvestigate lubricant loss from surfaces under flow in water usingboth quantitative analysis and visualization, testing the effectsof underlying surface type (nanostructured versus flat), as well asflow rate in the physiologically relevant range, lubricant type, andtime. We find lubricant losses on the order of only ng/cm2in a closed system, indicating that these interfaces are relativelystable under the flow conditions tested. No notable differences emergedbetween surface type, flow rate, lubricant type, or time. However,exposure of the lubricant layers to an air/water interface did significantlyincrease the amount of lubricant removed from the surface, leadingto disruption of the layer. These results may help in the developmentand design of materials using surface-immobilized lubricant interfacesfor repellency under flow conditions. [ABSTRACT FROM AUTHOR]