Preterm premature rupture of membranes eventuate when the amniotic sac breaks at least 4 weeks before a pregnancy has reached full term. The motive of this study was to evaluate the effect of preterm premature rupture of membranes on neonatal outcome and to establish correlations between mother's infection and gestational age, weight as well as hospitalization days of the newborn. A retrospective study was conducted over a period of 1 year in Bega Clinic of Obstetrics-Gynecology and Neonatology Timisoara. The study included 36 premature newborns admitted to Neonatal Intensive Care Unit, who meet the inclusion criteria. Confirming the diagnosis of neonatal sepsis includes the history of the neonate, clinical signs, symptoms and paraclinical investigations. The present work focuses on the history of mothers with PPRM over 16 hours, with or without infection, which indicates the need to look up for a possible neonatal infection. For laboratory tests: complete blood count, C-reactive protein, blood and vernix cultures were taken. Further research will analyze new markers of infection. Corroborating new findings with the data taken so far, helps us developing a novel clinical protocol and to improve the therapeutic management of this cases. [ABSTRACT FROM AUTHOR]