Molecular Methods to Measure Intestinal Bacteria: A Review.
- Resource Type
- Article
- Authors
- Douglas Inglis, G.; Thomas, Matthew C.; Thomas, Dallas K.; Kalmokoff, Martin L.; Brooks, Stephen P. J.; Brent Selinger, L.
- Source
- Journal of AOAC International. Jan/Feb2012, Vol. 95 Issue 1, p5-23. 19p. 1 Black and White Photograph, 4 Diagrams, 1 Chart, 5 Graphs.
- Subject
- *BACTERIA
*FLOW cytometry
*CYTOMETRY
*POLYMERASE chain reaction
*POLYMERIZATION
- Language
- ISSN
- 1060-3271
The article presents a study about the molecular methods for the measurement of intestinal bacteria. It mentioned the strengths and weaknesses of the methods. It utilized the advantage of terminal restriction fragment length polymorphism (T-RFLP) to characterize the communities in all samples. It also noted the application of flow cytometry fluorescent in situ hybridization (FCM-FISH) and quantitative polymerase chain reaction (qPCR) in the quantification of groups of bacteria.