Abbreviations & Acronyms DNA deoxyribonucleic acid FFPE formalin-fixed paraffin-embedded HE hematoxylin and eosin HPV human papillomavirus PCR polymerase chain reaction PeIN penile intraepithelial neoplasia pSCC penile squamous cell carcinoma pSCC belongs to a group of HPV-related cancers.1 Recently, a major systematic review with meta-analysis reported a pooled HPV DNA prevalence rate of 50.8% in penile cancer.2 In the present study, we analyzed tissue from a large well-characterized cohort of Danish pSCC patients to assess HPV prevalence, p16INK4a positivity, and important histopathological features of penectomy specimens. Sixty-eight patients (42%) presented with phimosis, and HPV-negative patients were less likely to present with phimosis compared to HPV-positive patients (26/79 [33%] I vs i 42/84 [50%]; I P i = 0.02). Of the 84 HPV-positive patients, 61 (73%) tested positive for a single HPV subtype and therefore HPV positivity with a single HPV subtype was the most frequent pattern of HPV infection. [Extracted from the article]