Improved production, characterization and flocculation properties of poly (γ)-glutamic acid produced from Bacillus subtilis.
- Resource Type
- Article
- Authors
- Bhunia, B.; Mukhopadhy, D.; Goswami, S.; Mandal, T.; Dey, A.
- Source
- Journal of Biochemical Technology. 2012, Vol. 3 Issue 4, p389-394. 6p. 6 Color Photographs.
- Subject
- *BACILLUS subtilis
*POLYSACCHARIDES
*BIOPOLYMERS
*GLUTAMIC acid
*UMAMI (Taste)
*GLYCERIN
- Language
- ISSN
- 0974-2328
Bacillus subtilis 2063 produced extracellular biopolymer which showed excellent flocculation activity. The biopolymer was confirmed as poly (γ-glutamic acid) (PGA) by using product characterization. HPLC profile showed that molecular weight of PGA was found to be 5.8×106 Da. Improved production, Characterization and flocculation properties of PGA produced by Bacillus species were studied. PGA produced by B. subtilis was devoid of any polysaccharides. The flocculating activity was markedly stimulated by the addition of cations. The pH of reaction mixture also influenced the flocculating activity. Glycerol and ammonium chloride were found to be most useful carbon and nitrogen sources. An overall 4.24-fold increase in protease production was achieved in the design medium composed with Glycerol and ammonium chloride as a carbon and nitrogen sources as compared with basal media. PGA production increased significantly with optimized medium (21.42 gl-1) when compared with basal medium (5.06 gl-1) [ABSTRACT FROM AUTHOR]