Synthesis and Applicability of Base-Discriminating DNA-Triplex-Forming 19F NMR Probes.
- Resource Type
- Article
- Authors
- Bhuma, Naresh; Tähtinen, Ville; Virta, Pasi
- Source
- European Journal of Organic Chemistry. 2/7/2018, Vol. 2018 Issue 5, p605-613. 9p.
- Subject
- *QUADRUPLEX nucleic acids
*MOLECULAR structure
*NUCLEAR magnetic resonance
*HYDROGEN bonding
*NUCLEIC acid synthesis
- Language
- ISSN
- 1434-193X
Phosphoramidite building blocks of CF3-modified N4(6-amino-2-pyridinyl)deoxycytidines were synthesized, incorporated into triplex-forming 2'-deoxyoligonucleotide strands and the applicability of the probes to recognize nucleobase content in the pyrimidine-rich strand of double-helical DNA targets was evaluated. As expected, the obtained 19F NMR resonances were sensitive to the base content and unique 19F NMR spectral fingerprints could be obtained. [ABSTRACT FROM AUTHOR]