Platform for in situ real-time measurement of protein-induced conformational changes of DNA.
- Resource Type
- Article
- Authors
- Spuhier, Philipp S.; Kneževuc, Jelena; Yalçin, Ayca; Bao, Qiuye; Pringsheim, Erika; Dröge, Peter; Rant, Ulrich; ÜnIü, M. Selim
- Source
- Proceedings of the National Academy of Sciences of the United States of America. 1/26/2010, Vol. 107 Issue 4, p1397-1401. 5p.
- Subject
- *CONFORMATIONAL analysis
*ESCHERICHIA coli
*PROTEIN binding
*DNA microarrays
*LITHOGRAPHY
*BIOSENSORS
- Language
- ISSN
- 0027-8424
A platform for in situ and real-time measurement of proteininduced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia co/i integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay. [ABSTRACT FROM AUTHOR]