Systemic inflammatory response to surgical interventions has been characterized by an orchestrated interplay of endogenous mediatory cytokines and haematologic alterations. The study was carried out to determine the changes in plasma levels of pro-inflammatory interleukin-6 (IL-6) and anti-inflammatory interleukin-10 (IL-10) in relation to haematological alterations in response to colo-peritoneo-cystoplasty in dogs. Haematological and plasma concentrations of IL-6 and IL-10 were profiled in four apparently healthy dogs, following the colo-peritoneo-cystoplastic procedure. Mean packed cell volume (PCV) significantly (P < 0.05) decreased from the pre-surgical 45.84 ± 3.67 to a lowest value (33.50 ± 2.53%) on day 7 post-surgery. Mean total leukocyte count (TLC) significantly (P < 0.05) increased from the pre-surgical value (9.85 ± 1.02 × 109/L) to a peak level (14.20 ± 2.77 × 109/L) on day 7 post-surgery. Mean neutrophil count significantly (P < 0.05) increased from the pre-surgical value of 6.63 ± 0.79 to a peak day 7 post-surgical value (10.00 ± 2.39 × 109/L. Mean lymphocyte count rose from 3.59 ± 0.31 to day 4 post-surgical value of 4.42 ± 0.82 × 109/L. Changes in mean eosinophil and monocyte counts were not significant (P > 0.05). Mean plasma IL-6 concentration significantly (P < 0.05) increased from 7639.45 ± 146.81 (pre-surgical) to 7916.62 ± 32.97 pg/mL on day 4 post-surgery. The pre-surgical mean IL-10 concentration was 93.95 ± 17.18 pg/mL. It remained relatively unchanged post-surgery until on day 9 when it suddenly surged to 449.70 ± 125.79 pg/mL. The mean concentration of the IL-10 slightly decreased and fluctuates until day 35 when another peak rise (457.20 ± 95.78 pg/mL) was observed. It was observed that changes in plasma concentrations of IL-6 and IL-10 positively correlated with the leukocytic changes, which suggest presence of a regulatory interplay of IL-6, IL-10 and leukocytic changes in wound healing inflammatory response to the colo-peritoneo-cystoplasty in dogs. [ABSTRACT FROM AUTHOR]