This study evaluated the distribution and size of lipid droplets (LDs) in oocytes recovered from young and adult ovine ovaries. Collected oocytes were categorised on the basis of their major diameter (small (SO), 70–90 µm; medium (MO), >90–110 µm; large (LO), >110–130 µm) and were stained with Nile red to detect LDs. In adult and young oocytes, a diffuse pattern distribution of LDs was dominant in all classes except adult LO and young SO and LO. Larger LDs (i.e. >3 µm) were mostly present in young SO and LO, whereas smaller LDs (1–3 µm) were detected in the other adult and young oocyte categories. Information about changes in lipid accumulation during oocyte growth phases and the localisation of lipids in the cytoplasm is scarce. This study evaluated the morphological distribution and size of lipid droplets in young and adult ovine oocytes recovered during the growing phases. The findings of this study support the metabolic activities that take place during folliculogenesis. [ABSTRACT FROM AUTHOR]