Molecular Exchange between Intra- and Extracellular Compartments in a Cell Suspension.
- Resource Type
- Article
- Authors
- Åslund, Ingrid; Nowacka, Agnieszka; Nilsson, Markus; Topgaard, Daniel
- Source
- AIP Conference Proceedings. Dec2008, Vol. 1081 Issue 1, p79-82. 4p. 1 Diagram, 2 Graphs.
- Subject
- *NUCLEAR magnetic resonance
*MAGNETIC resonance
*DIFFUSION
*SPECTRUM analysis
*CELL suspensions
- Language
- ISSN
- 0094-243X
In this work we present a PFG-SE technique to obtain the a characteristic time for molecular exchange between domains with slow diffusion and domains with fast diffusion. The method has been demonstrated by measuring the change of exchange time with temperature for a sedimented yeast cell suspension. Compared to previous methods the new protocol is more sensitive to exchange and as few as 40 data points are needed. The experimental time needed is thus a couple of minutes. [ABSTRACT FROM AUTHOR]