Influence of the 15-HETE on cytosolic [ Ca2+] i of the rabbit pulmonary artery smooth muscle cells
- Resource Type
- article
- Authors
- Tang, Xiaobo; Zhang, Rong; Wang, Zhigang; Yu, Lei; Li, Shanshan; Zhu, Daling
- Source
- Brazilian Archives of Biology and Technology. April 2008 51(2)
- Subject
- 15-hydroxyeicosatetraenoic acid
hypoxia
pulmonary artery rings
pulmonary artery smooth muscle cells
[ Ca2+]i
- Language
- English
- ISSN
- 1516-8913
Extracellular Ca2+ influx was blocked by L-type Ca2+ channel blocker nifedipine to observe the effects of 15-hydroxyeicosatetraenoic acid on the constriction of rabbit pulmonary artery rings and on the changes of Ca2+ level in the rabbit pulmonary artery smooth muscle cells, and further to investigate the mechanism of the calcium mobilization induced by the 15-HETE under hypoxic conditions. The effect of extracellular Ca2+ on tension of the rabbit PA rings was also studied. Nifedipine (10 µ mol/L) had no effect on 1 µ mol/L 15-hydroxyeicosatetraenoic acid induced vasoconstriction under normoxic and hypoxic conditions. Intracellular Ca2+ increased markedly in the 15-HETE group (cells were exposed to 1 µ mol/L 15-HETE for 8 min during culture) compared to the control group (P < 0.05). The study demonstrated that the 15-HETE could induce the elevation of Ca2+ in the pulmonary artery smooth muscle cells and the elevated calcium came from the release of the intracellular calcium.