The CCCH-type zinc finger proteins are a superfamily involved in many aspects of plant growth and development. However, the precise role of these proteins involved in plant stress tolerance are poorly understood. In this study, the regulatory and functional roles of two CCCH-type zinc finger protein, AtOZF1 (At2g19810) and AtOZF2 (At4g29190), under abiotic stress were investigated. More interesting, both of AtOZF1 and AtOZF2 protein were localized in the plasma membrane. The transcripts of AtOZF1 and AtOZF2 were highly induced by treatment with hydrogen peroxide, abscisic acid and salinity. The AtOZF1- or AtOZF2-overexpressing plants were relatively resistant to oxidative stress than WT and T-DNA insertion mutant atozf1 or antisense plant asozf2. Malondialdehyde, a decomposition product of lipid peroxidation, accumulated more in atozf1 mutant than in WT. Furthermore, atozf1 mutants displayed lower activities of catalase and guaiacol peroxidase, higher chlorosis, and down-regulated expression of antioxidant genes under oxidative stress. Unlike AtOZF1, loss function of AtOZF2 led to reduced root length, stem elongation and leaf size. It is shown that both AtOZF1 and AtOZF2 regulated ABA and salt responses in seed germination and cotyledon greening processes, because atozf1 and asozf2 were more sensitive to ABA and salt in each of these processes. By contrast, AtOZF1-or AtOZF2-overexpression plants were more resistant to ABA and salt stress than WT. In the presence of ABA and salinity, the transcript level of ABI2 but not ABI1 in AtOZF1- or AtOZF2-overexpression plant was lower than that in WT whereas the expression of ABI2 in both atozf1 and asozf2 was significantly enhanced. These results indicate that AtOZF1 and AtOZF2 regulate ABA and salt stress response through ABI2-mediated signal pathway. Taken together, these findings provide compelling evidence that AtOZF1 and AtOZF2 are important regulators for plant tolerance to abiotic stress.There are about 45 LecRKs in Arabidopsis, but only few of them have been studied. Here, we investigated the effect of a plasma membrane-localized L-type lectin-like protein kinase, AtLPK1 (At4g02410), disruption and overexpression upon plant responses to abiotic and biotic stress. The AtLPK1-overexpressing plant showed reduced ABA sensitivity in their cotyledon greening and root elongation. Furthermore, overexpression of AtLPK1 conferred transgenic plants tolerance to salt stress. In contrast, aslpk1 antisense plants were found to be more sensitive to ABA and salt stress during these processes. A group of ABA- and salt- responsive genes (RD29A, RD29B and COR15A) were elevated more in AtLPK1-overexpression plants than that in WT when exposure to ABA and salt treatment, whereas the expression of these genes in antisense plant had decreased compared with that in WT. On the other hand, AtLPK1-overexpression plant manifested increased resistance to pathogen Pseudomonas syringae pv. tomato (Pto) DC3000 and exhibited stronger expression of a group pathogenesis-related genes than WT. Our current data suggests therefore that AtLPK1, as a molecular link between ABA signaling and plant defense, functions at both abiotic and biotic stress tolerance.