Tannase (Tan410) from a soil metagenomic library was immobilized on different supports,including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and wasfurther characterized. The optimum pH of the immobilized Tan410 was shifted towardneutrality compared with the free enzyme (from pH 6.4 to pH 7.0). The optimum temperaturewas determined to be 45°C for the immobilized enzyme and 30°C for the free enzyme,respectively. The immobilized enzyme had no loss of activity after 10 cycles, and retainedmore than 90% of its original activity after storage for 30 days. After immobilization, theenzyme activity was only slightly affected by Hg2+, which completely inhibited the activity ofthe free enzyme. The immobilized tannase was used to remove 80% of tannins from a green teainfusion on the first treatment. The beads were used for six successive runs resulting in overallhydrolysis of 56% of the tannins.