Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae: a leap into disease pathophysiology
- Resource Type
- Brief Communication
- Authors
- Welle, Thijs; Hoekstra, Anna T.; Daemen, Ineke A. J. J. M.; Berkers, Celia R.; Costa, Matheus O.
- Source
- Metabolomics: An Official Journal of the Metabolomics Society. July 2017 13(7):1-7
- Subject
- Brachyspira
Swine dysentery
In vitro organ culture
Host-pathogen interactions
Pathophysiology
Nitric oxide
- Language
- English
- ISSN
- 1573-3882
1573-3890
Introduction:Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available.Objective:The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae.Methods:Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes.Results:Significant epithelial necrosis, increased levels of l-citrulline and IL-1α were observed on explants infected with B. hyodysenteriae.Conclusions:The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1α and NO.