Purpose: This study aimed to investigate the effects of gradually increasing treadmill inclination on the electroencephalogram(EEG) of stroke patients during gait training. Methods: Three stroke patients who were diagnosed with stroke within six months and capable of walking on a treadmill wereselected as subjects. EEG electrodes were attached at Fp1, Fp2, F3, F4, C3, C4, P3, and P4 positions of the cerebral hemispheresusing the International 10-20 system. The intervention involved walking for 2 minutes each at 0 degrees, 15 degrees, and 30 degreesinclination on the treadmill while focusing on a target point located in front during the treadmill gait training. The EEG(Smartingmobi, Serbia) generated when the treadmill gradient gradually increased was measured. In addition, relative alpha andrelative beta waves were visualized through the Brain mapping program in the TeleScan program to assess the changes in eachbrain region for the activity of the EEG. Results: The relative alpha wave value decreased as treadmill inclination increased, while the relative beta wave value increased. Conclusion: Gradually increasing the inclination during treadmill gait training appears to be a crucial parameter forincreasing the brain activity levels of stroke patients.