Single cell isolation and pick up from the root tip of Arabidopsis
- Resource Type
- Journal Article
- Authors
- Bilal TURAN; Di CHANG; Fumihito ARAI; Yoshikatsu SATO; Yuko UKAI
- Source
- The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec). 2022, :2-N08
- Subject
- Arabidopsis
microfluidic chip
piezo pump
- Language
- English
- ISSN
- 2424-3124
Arabidopsis is a kind of model plant for studying the mechanism of plant growth and development including photosynthesis and environmental stress response. Lots of studies on Arabidopsis requires the analysis of the root tip. Previously, biologists always cut the root tip and put them in special chemical to isolate the protoplast and analyze the RNA or protein of the cells. But this method has a disadvantage. Protoplast are exposed to enzyme solution long time after they are isolated. This might disturb the original gene expression in the protoplast and affect the result of transcriptome result. In this paper, the author proposed a system combining a microfluidic chip and a micromanipulator with a piezo pump which can be used to isolate and pick up a single cell from the root tip so that the protoplast can be picked up shortly after it is isolated. Thus, it would help to acquire transcriptome data that is close to the original expression. This system would benefit the study of Arabidopsis in the future.