Background. A 19-gauge needle has recently become available for use in endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). It is expected to be possible to obtain a larger size of specimens using this needle compared to those available previously. Case. A 69-year-old man underwent right ophthalmectomy under a diagnosis of right choroid malignant melanoma 4 years previously. Three years ago, a nodule measuring 23 mm in longest dimension developed in the superior segment of the lower lobe of the right lung, but it was negative on positron emission tomography-computed tomography (PET-CT), and so he was followed up with chest CT. One-and-a-half years ago, we performed EBUS-TBNA using a 22-gauge needle for diagnosis, but there were no specific findings. Subsequently, the lesion showed slight enlargement, and we again performed EBUS-TBNA with a 19-gauge needle to obtain a larger specimen. A total of four passes were made, and we obtained histological specimens with cartilage suggesting pulmonary hamartoma. Conclusions. In the present case, EBUS-TBNA using a new 19-gauge needle provided larger and better quality specimens compared to the conventional 22-gauge needle, and was effective for diagnosing pulmonary hamartoma.