This study aims to explore the digital citizenship education of pre-service teachers through deriving the needs for digital citizenship of pre-service teachers and analyzing their perceptions. To this end, an online survey was conducted on pre-service teachers enrolled in a graduate school of education, and data from 245 respondents were collected and analyzed. The needs assessment was prioritized by synthesizing paired t-test, Borich needs assessment, and the locus for focus model. The results of the study were as follows; first, when synthesizing the analysis of needs, the priority of digital citizenship of pre-service teachers was 'self-protection'. Second, pre-service teachers were aware of the need for digital citizenship education and were willing to participate in it. They hoped that it would be taught in an extra-curricular program or teacher education courses. Based on the study results, the direction of education to strengthen the digital citizenship of pre-service teachers was discussed.