对硫酸软骨素纳米硒(selenium-chondroitin sulfate nanoparticles,SeCS)的结构进行鉴定,并考察其对Hela细胞迁移和侵袭的影响.以硫酸软骨素为模板,采用亚硒酸钠-维生素C氧化还原法制备SeCS,并运用透射电镜、扫描电镜、X射线光电子能谱仪和傅立叶红外光谱仪对SeCS进行结构表征;通过划痕实验和细胞迁移侵袭实验(Transwell)检测SeCS对Hela细胞迁移及侵袭的影响;通过Western blot免疫印迹法检测SeCS对细胞内基质金属蛋白酶-2(matrix metalloproteinase 2,MMP-2)和基质金属蛋白酶-9(matrix metalloproteinase 9,MMP-9)表达水平的影响.实验结果表明,制备所得的SeCS是零价态的分散良好的球形纳米粒,表面光滑.Hela细胞经20 μg/mL SeCS处理后,细胞的迁移率和侵袭率分别从对照组的100%下降到(59.19±7.74)%和(82.43± 4.21)%,表明SeCS能够抑制细胞的迁移和侵袭.进一步的研究发现,SeCS下调了 MMP-2和MMP-9蛋白的表达量,表明SeCS通过降低MMP-2和MMP-9的蛋白表达来抑制细胞的迁移和侵袭.
This study aimed to identify the structure of selenium-chondroitin sulfate nanoparticles(SeCS)and investigate its effect on the migration and invasion of Hela cells.SeCS were prepared by the sodium selenite(Na2SeO3)and ascorbic acid redox reaction using chondroitin sulfate as a template and characterized by transmission electron microscopy(TEM),scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),and Fourier transform infrared spectroscopy(FTIR).The effects of SeCS on the migration and invasion of Hela cells were detected by scratch test and cell migration and invasion assay(Transwell).The effects of SeCS on matrix met-alloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)expression levels were detected using western blot.Results showed that the prepared SeCS were zero-valent spherical nanoparticles with a smooth surface.After Hela cells were treated with 20 μg/mL SeCS,the migration and invasion rates of cells were decreased from 100%in the control group to(59.19±7.74)%and(82.43±4.21)%,respectively,indicating that SeCS could inhibit cell migration and invasion.Further studies found that SeCS down-regulated the expression levels of MMP-2 and MMP-9 proteins,indicating that SeCS inhibited cell migration and invasion by reducing the expression of MMP-2 and MMP-9 proteins.