目的 探索多巴胺包裹的纳米金粒子(Au@PDA)介导的光热治疗对黑色素瘤生长的抑制作用.方法 制备用于光热治疗的球形纳米金粒子(Au)和 Au@PDA;体外分别通过 CCK-8、划痕实验、Annexin V/PI 双染法检测Au@PDA介导的光热治疗对细胞增殖、迁移和凋亡的影响;通过检测光照后 HMGB1 分泌情况,验证光热治疗对细胞免疫性的影响;通过动物实验验证 Au@PDA介导的光热治疗对肿瘤生长、机体免疫的激活情况;最后通过 HE 染色观察纳米粒子对各主要脏器的影响.结果 成功地制备了形貌均一的球形的Au和Au@PDA,Au@PDA在 808 nm激光照射后,能显著诱导细胞凋亡,抑制细胞的增殖和迁移,促进细胞分泌 HMGB1;动物实验结果显示 Au@PDA光照后能显著地导致肿瘤组织坏死、增加肿瘤组织中浸润T淋巴细胞数量,激活机体抗肿瘤免疫,抑制肿瘤生长.结论 Au@PDA介导的光热治疗,不仅可以通过直接的热能杀伤肿瘤细胞,还可以激活机体的抗肿瘤免疫反应,从而抑制肿瘤的生长.
Objective To explore inhibition of melanoma growth by photothermal therapy mediated with dopa-mine-coated gold nanoparticles(Au@PDA).Methods Firstly,spherical gold nanoparticles(Au)and Au@PDA were prepared for photothermal therapy(PTT).Cell survival rate was test by CCK-8,cell migration was measured by scratch test,and the apoptosis was detected by Annexin V/PI staining in vitro.The secretion of HMGB1 from cells after photo-thermal therapy was measured by ELISA to confirm the influence on tumor cell immunity.Further,the tumor inhibition and anti-tumor immune response activation by Au@PDA mediated PTT were observed in vivo.Finally,the effects of nanoparticles on the major organs were observed by HE staining.Results The Au and Au@PDA were both successfully prepared.Au@PDA could significantly induce cell apoptosis,inhibit cell proliferation and migration,increase the secre-tion of HMGB1 after irradiated with 808nm laser.The animal experiment results showed that the photothermal therapy mediated with Au@PDA could significantly lead tumor tissue necrosis,increase the number of infiltration T cell in tumor tissue,active the anti-tumor immune response,and finally inhibit tumor growth.HE staining results showed the nanop-articles had no obvious damage to the main organ.Conclusion Photothermal therapy mediated by Au@PDA can not only kill tumor cells through directly heat energy,but also activate the anti-tumor immune response to inhibit tumor growth.