目的:探讨医院制剂鱼芩解毒丸的体外抗HIV-1病毒活性.方法:采用MTT比色法检测鱼芩解毒丸的细胞毒性;采用细胞病变法检测鱼芩解毒丸对HIV-1急性感染的抑制活性;采用HIV-1ⅢB慢性感染细胞的p24抗原表达抑制实验检测鱼芩解毒丸的抗H1V-1活性.结果:鱼芩解毒丸抑制HIV-1ⅢB诱导C8166细胞形成合胞体的EC50为0.035 ~0.070 mg· mL-1,治疗指数为42.79~57.03 mg·mL-1;鱼芩解毒丸抑制HIV-1ⅢB感染C8166的p24抗原表达的EC50为0.070 ~0.090 mg· mL-1,治疗指数22.82~28.51 mg·mL-1.结论:鱼芩解毒丸具有一定的抗HIV-1活性.
Objective:To assess the anti-HIV-1 activity of a hospital made pill named yu qin jie du pill in vitro.Methods:To detect cytotoxicity of the yu qin jie du pill by MTT staining method;to detect inhibitory activity of yu qin jie du pill in acute HIV-1 infection by detecting cell lesions;to detect anti HIV-1 activity of yu qin jie du pill by inhibition of P24 antigen on HIV-1 Ⅲ B cells of chronic infection.Results:The 50% effective concentration (ECso) of yu qin jie du pill inhibition of HIV-1 Ⅲ B induced C8166 cells to form syncytia was 0.035-0.070 mg · mL-1,treatment index was 4.2.79 ~57.03 mg · mL-1;The 50% effective concentration (EC50) of yu qin jie du pill inhibition of HIV-1 Ⅲ B infected C8166 P24 antigen expression was 0.070 ~ 0.090 mg · mL-1,and the therapeutic index was 22.82 ~28.51 mg · mL-1.Conclusion:Yu qin jie du pill had certain anti-HIV-1 activity.