Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
- Resource Type
- Authors
- Ping Jiang; Linlin Fang; Xing Liu; Yanni Gao; Xianwei Wang; Juan Bai
- Source
- Veterinary Research, Vol 52, Iss 1, Pp 1-13 (2021)
Veterinary Research
Veterinary Research, BioMed Central, 2021, 52 (1), pp.52. ⟨10.1186/s13567-021-00922-0⟩
- Subject
- 0301 basic medicine
Gene Expression Regulation, Viral
animal diseases
viruses
Sus scrofa
Pseudorabies
Virus Replication
Virus
Long non-coding RNAs (lncRNAs)
Cell Line
Pseudorabies virus
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Animals
Gene
JAK/STAT1
Host factor
lcsh:Veterinary medicine
General Veterinary
biology
Anti-viral activity
RNA
virus diseases
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Virology
Herpesvirus 1, Suid
Long non-coding RNA
3. Good health
030104 developmental biology
Viral replication
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA, Viral
lcsh:SF600-1100
RNA, Long Noncoding
IFN-alpha
030217 neurology & neurosurgery
Research Article
- Language
- English
- ISSN
- 1297-9716
0928-4249
Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection.