Additional file 1 of SMN-deficiency disrupts SERCA2 expression and intracellular Ca2+ signaling in cardiomyocytes from SMA mice and patient-derived iPSCs
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- Guzal Khayrullina; Moritz, Kasey E.; Schooley, James F.; Fatima, Naheed; Viollet, Coralie; McCormack, Nikki M.; Smyth, Jeremy T.; Doughty, Martin L.; Dalgard, Clifton L.; Flagg, Thomas P.; Burnett, Barrington G.
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Additional file 1:Supplemental Figure 1. Elevated expression of ANP in SMA model mice. qRT-PCR analysis of ANP mRNA expression. Expression levels (mean ± SEM) in SMN-deficient (SMA) heart tissue are represented relative to unaffected controls at each time point. Statistical analysis: p values shown for one-way ANOVA, Tukey’s post hoc. Supplemental Figure 2. Serca2 levels reduced in cardiomyocytes derived from SMN-deficient iPSCs. Expression of SERCA2a expression was determined in control and SMN-deficient iPSC-derived cardiomyocytes. Cells were transfected with siRNA targeting SMN on day 18 of differentiation and qRT-PCR performed 48 hours after transfection. Values represent mean ± SEM.