Mapping of Interactions between Human Macrophages and Staphylococcus aureus Reveals an Involvement of MAP Kinase Signaling in the Host Defense
- Resource Type
- Authors
- Joerg Bernhardt; Doerte Becher; Jan Maarten van Dijl; Malgorzata Miller; Andreas Otto; Maikel P. Peppelenbosch; Annette Dreisbach; Michael Hecker
- Source
- Journal of Proteome Research, 10(9), 4018-4032. American Chemical Society
Journal of Proteome Research, 10(9), 4018-4032. NLM (Medline)
Journal of Proteome Research, 10, 9, pp. 4018-32
Journal of Proteome Research, 10, 4018-32
- Subject
- Staphylococcus aureus
HISTONE H1
MAP Kinase Signaling System
media_common.quotation_subject
Host–pathogen interaction
Blotting, Western
RECEPTOR-MEDIATED PHAGOCYTOSIS
TYROSINE KINASE
Human pathogen
host-pathogen interaction
Biology
medicine.disease_cause
Proteomics
Biochemistry
Microbiology
CYTOPLASMIC DOMAIN
SDG 3 - Good Health and Well-being
Bacterial Proteins
Heat shock protein
Protein Interaction Mapping
medicine
HEAT-SHOCK PROTEINS
Humans
Phosphorylation
Internalization
media_common
TOLL-LIKE RECEPTORS
NITRIC-OXIDE
Macrophages
JNK Mitogen-Activated Protein Kinases
NEUTROPHIL EXTRACELLULAR TRAPS
EPITHELIAL-CELLS
General Chemistry
Neutrophil extracellular traps
Actins
Cell biology
Pathogenesis and modulation of inflammation [N4i 1]
Gene Expression Regulation
kinase profiling
Host-Pathogen Interactions
Microscopy, Electron, Scanning
PHOSPHATIDYLINOSITOL 3-KINASE
Mitogen-Activated Protein Kinases
Signal transduction
signal transduction
- Language
- ISSN
- 1535-3907
1535-3893
Item does not contain fulltext Staphylococcus aureus is a dangerous opportunistic human pathogen that causes serious invasive diseases when it reaches the bloodstream. Recent studies have shown that S. aureus is highly resistant to killing by professional phagocytes and that such cells even provide a favorable environment for intracellular survival of S. aureus. Importantly, the reciprocal interactions between phagocytes and S. aureus have remained largely elusive. Here we have employed kinase profiling to define the nature and time resolution of the human THP-1 macrophage response toward S. aureus and proteomics to identify the response of S. aureus toward macrophages. The results of these studies reveal major macrophage signaling pathways triggered by S. aureus and proteomic signatures of the responses of S. aureus to macrophages. We also identify human proteins bound to S. aureus that have potential roles in bacterial killing and internalization. Most noticeably, our observations challenge the classical concept that macrophage responses are mainly mediated through Toll-like receptor 2 and NF-kappaB signaling and highlight the important role of the stress-activated MAP kinase signaling in orchestrating the host defense.