T follicular helper (T(FH)) cells are crucial for effective humoral immunity by providing the required signals to cognate B cells and promoting germinal center (GC) formation. Many intrinsic and extrinsic factors have been reported to be involved in the multistage, multifactorial differentiation process of T(FH) cells. By comparing gene expression between T(FH) cells and T(H)1 cells based on published GEO data, we found selective and high expression of sclerostin domain-containing protein 1 (SOSTDC1) in T(FH) cells but not in T(H)1 cells; however, it is unclear whether SOSTDC1 is important for the differentiation and/or function of T(FH) cells. Using a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection, we confirmed the selective expression of SOSTDC1 in T(FH) cells compared to that in T(H)1 cells, but the ablation of SOSTDC1 did not affect T(FH) cell differentiation or effector function. Thus, our results indicate that the SOSTDC1 protein is merely a specific marker of T(FH) cells but does not play a functional role in the differentiation of T(FH) cells during acute viral infection.