Nanosilicate embedded agarose hydrogels with improved bioactivity
- Resource Type
- Authors
- Ali Nadernezhad; Bahattin Koc; Özüm Şehnaz Çalışkan; Yusuf Z. Menceloğlu; Fuat Topuz
- Source
- Carbohydrate polymers. 201
- Subject
- Polymers and Plastics
02 engineering and technology
010402 general chemistry
01 natural sciences
Nanocomposites
chemistry.chemical_compound
Mice
Rheology
Materials Testing
Materials Chemistry
medicine
Animals
Fourier transform infrared spectroscopy
Fibroblast
Cell Proliferation
Nanocomposite
Chemistry
Sepharose
Silicates
Organic Chemistry
Hydrogels
Cells, Immobilized
021001 nanoscience & nanotechnology
0104 chemical sciences
medicine.anatomical_structure
Chemical engineering
Nanofiber
Self-healing hydrogels
NIH 3T3 Cells
Agarose
Swelling
medicine.symptom
0210 nano-technology
- Language
- ISSN
- 1879-1344
This paper reports the synthesis of nanocomposite agarose hydrogels with improved bioactivity with the incorporation of anisotropic 2D nanosilicates (Laponite) to promote cell binding, growth and proliferation. Rheological measurements showed that the incorporation of nanosilicates slightly increased the gelation temperature (T-gel). The use of higher nanosilicate content at the constant agarose concentration improved the mechanical properties of the gels. Due to the non-swelling nature of agarose, the addition of nanosilicates did not result in any remarkable change in the swelling properties of the agarose gels, while collapsed agarose nanofibers were observed with the incorporation of nanosilicates. EDX analysis confirmed the presence of the embedded nanosilicates in the gel matrix. The existence of physical interactions between nanosilicate and agarose was demonstrated by FTIR over the shifting of Si-O stretching band to a lower frequency. The encapsulated NIH/3T3 fibroblast cells showed enhanced proliferation and spreading in the presence of nanosilicates.