Engineering of Cry3Bb1 provides mechanistic insights toward countering western corn rootworm resistance
- Resource Type
- Article
- Authors
- Kuwar, Suyog S.; Mishra, Ruchir; Banerjee, Rahul; Milligan, Jason; Rydel, Timothy; Du, Zijin; Xie, Zhidong; Ivashuta, Sergey; Kouadio, Jean-Louis; Meyer, Jason M.; Bonning, Bryony C.
- Source
- Current Research in Insect Science; January 2022, Vol. 2 Issue: 1
- Subject
- Language
- ISSN
- 26665158
•Cry3Bb1 engineering for receptor retargeting to counter WCR resistance described•Phage display screens against BBMV or recombinant insecticidal-protein receptors•77 gut binding peptides selected and engineered into 284 new variants of Cry3Bb1•112 variants were active against susceptible but not resistant WCR•Engineering of Cry3Bb1 Domain II loops 1 and 2 disrupted insecticidal activity•Sites for Cry3Bb1 engineering and implications for Cry3Bb1 resistance discussed