We have examined the intracellular route, coenzyme conversion and transcytosis rate of [57 Co]-labeled cobalamin (Cbl) in function of its presentation to the apical side of Caco-2 cells, either free or bound to intrinsic factor (IF). The free-presented Cbl was progressively bound to endogenous transcobalamin II (TCII) which may stem, in part, from a basolateral to apical passage. Its transcytosis was TCII-mediated as it was abolished when antibodies to TCII were added to the apical medium. The apparent permeability coefficient (Papp) was estimated at 20.8±3.6, 103.5±17.7, 0.9±0.3 × 10–5 cm/h for TCII-Cbl, IF-Cbl and haptocorrin-Cbl, respectively. Chloroquine inhibited the transcytosis rate of both TCII and IF-bound Cbl in a dose-dependent manner. Approximately 80% of apical Cbl, bound to either exogenous IF or endogenous TCII, was transported to the basolateral side as intact cyano[57Co]Cbl whereas the remainder was converted into Ado-Cbl and CH3-Cbl within the cells, as shown by HPLC analyses of a 1,000-gpellet and a 12,000-gsupernatant. Coenzymatic conversion was virtually abolished by chloroquine. In conclusion, we suggest that apically presented free Cbl is internalized viaTCII-dependent transport. The apically internalized CN-Cbl, bound to either IF or TCII, is processed viaan acidic vesicle and part of it is converted to coenzymes, whereas bulk of CN-Cbl is transcytosed intact.