High-Speed, Whole-Column Fluorescence Imaging Detection for Isoelectric Focusing on a Microchip Using an Organic Light Emitting Diode as Light Source.
- Resource Type
- Article
- Authors
- Bo Yao; Huihua Yang; Qionglin Liang; Guoan Luo; Liduo Wang; Kangning Ren; Yudi Gao; Yiming Wang; Yong Qiut2,3
- Source
- Analytical Chemistry. 8/15/2006, Vol. 78 Issue 16, p5845-5850. 6p. 1 Diagram, 6 Graphs.
- Subject
- *CHEMICAL research
*FLUORESCENCE spectroscopy
*ISOELECTRIC focusing
*FLUORIMETRY
*LIGHT emitting diodes
*LIGHT sources
*INTEGRATED circuits
*ELECTROLYTES
*ELECTRIC fields
- Language
- ISSN
- 0003-2700
An integrated and simplified microfluidic device using a 250 μm × 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carder for IEF. Under optimized IEF conditions, the detection limit of this system was ∼0.6 μg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems. [ABSTRACT FROM AUTHOR]