Identification of a rare subset of adipose tissue-resident progenitor cells, which express CD133 and TRPC3 as a VEGF-regulated Ca2+ entry channel
- Resource Type
- Article
- Authors
- Poteser, Michael; Graziani, Annarita; Eder, Petra; Yates, Ameli; Mächler, Heinrich; Romanin, Christoph; Groschner, Klaus
- Source
- FEBS Letters. Aug2008, Vol. 582 Issue 18, p2696-2702. 7p.
- Subject
- *ADIPOSE tissues
*CALCIUM channels
*VASCULAR endothelial growth factors
*CELLS
*PROTEINS
*CALCIUM ions
- Language
- ISSN
- 0014-5793
Abstract: VEGF-induced Ca2+ signalling was investigated in CD133+/VEGFR-2+ progenitor cells isolated from human adipose stroma. Colonies derived from CD133+ immunoselected cells displayed inhomogenous Ca2+ signals, with variable magnitude of VEGF-induced Ca2+ entry, which positively correlated with expression of the Ca2+ channel protein TRPC3. High levels of VEGF-induced Ca2+ entry and TRPC3 expression were preferentially detected in rim areas of expanding colonies. Dominant negative suppression of TRPC3 inhibited VEGF-induced Ca2+entry into CD133+ cells. Our results identify TRPC3 as a key Ca2+ entry channel in a subset of CD133+ stem cells. We suggest TRPC3 as an essential determinant of cell fate in CD133+ progenitor-derived colonies. [Copyright &y& Elsevier]