No Taste for Methyl: Methylation Sensitive Proteolysis in Drug Screening
- Resource Type
- Article
- Authors
- Bissinger, Elisabeth-Maria; Jung, Manfred
- Source
- Chemistry & Biology. Jul2010, Vol. 17 Issue 7, p677-678. 2p.
- Subject
- *DRUG use testing
*METHYLATION
*PROTEOLYSIS
*METHYLTRANSFERASES
*GENE therapy
*ENZYME inhibitors
*ELECTROPHORESIS
- Language
- ISSN
- 1074-5521
Methyltransferases and demethylases are emerging targets for epigenetic therapy. present a new assay for the enzyme inhibition of methyltransferases and demethylases based on selective enzymatic cleavage of unmethylated versus methylated peptides and their subsequent electrophoretic separation. [Copyright &y& Elsevier]