Introduction: Black Cumin Seed (Nigella sativa) is known to have anti-inflammatory activity because it contains thymoquinone. Manufacturing in the form of nanoemulsion extracts can accelerate the absorption process in the body. A Nanoemulsion is a transparent or translucent system with a uniform and very small globule size (usually in the 2-500 nm range). Nanoemulsions consist of surfactants, co surfactants, oil and water. Tween 80 is used as a surfactant, which when combined with VCO, will form a small nanoemulsion globule size (2-500 nm). PEG 400 act as a co surfactant to help lower interfacial tension. This study aims to determine the concentration of VCO, tween 80, and PEG 400, which can be formulated as nanoemulsion preparations. Methods: The preparation of black cumin extract nanoemulsion was carried out by a combination of the low energy emulsification method with a magnetic stirrer and the high energy emulsification method with a sonicator. Results: The results showed the test results droplet size nanoemulsion extract cumin, a black in colour with a concentration of 1 % produced a grain size of 139.3 nm, at a concentration of 2% obtained the particle size of 122.7 nm and at a concentration of 3% obtained the particle size of 154.8 nm. Conclusion: The polydisperse index is in the range of 0.57-0.59, which shows particle size uniformity nanoemulsi, because all three formulations have a similar polydisperse index, which means that the distribution of particles with particle size uniformity levels were very good. [ABSTRACT FROM AUTHOR]