The transcription factor Sox2 is essential for stem cells and adult progenitor cells to maintain the pluripotency of embryonic stem cells for tooth development both reptiles and mammals. However, little is known about Sox2 expression during tooth development in amphibian. The purpose of this study is to compare the expression of Sox2 and Shh in tooth development of Xenopus by using H-E staining and in situ hybridization. At stage 55, Sox2 and Shh were weakly expressed in the dental epithelium. By stage 57, tooth germ was the bell stage, the expressions of Sox2 and Shh were observed in the inner enamel epithelium. From stage 58, Sox2 and Shh were expressed in the inner enamel epithelium, and Sox2 was weakly expressed in the dental papilla. At stage 59, the expression pattern of Sox2 and Shh were similar to the previous stage, but Sox2 was strongly expressed in the dental papilla. At stage 62 and 64, the expression of Sox2 and Shh were observed in ameloblasts and odontoblasts. Taken together, these results indicate that Sox2 can be a dental lamina maker. These findings suggest that Sox2 may play a role in the dentinogenesis of tooth development in Xenopus.Keywords: Sox2, Shh, Ameloblast, Odontoblast, Xenopus laevis