Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori
- Resource Type
- Academic Journal
- Authors
- Osaki, Takako; Okuda, Masumi; Ueda, Junko; Konno, Mutsuko; Yonezawa, Hideo; Hojo, Fuhito; Yagyu, Kiyoko; Lin, Yingsong; Fukuda, Yoshihiro; Kikuchi, Shogo; Kamiya, Shigeru
- Source
- Journal of Medical Microbiology. May 01, 2013 62(5):761-765
- Subject
- Language
- English
- ISSN
- 0022-2615
This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family.