A sub-THz second harmonic multi-frequency gyrotron with Gaussian beam output, Gyrotron FU CW GVII, has been developed. This gyrotron was designed to change the frequency stepwise in the range from 270 to 420 GHz. Oscillations of seven second-harmonic modes were successfully observed and Gaussian beam radiations converted from six oscillation modes were observed. Two second harmonic modes; TE 6,5 and TE 8,5 ; oscillated in a single mode. Even in case of simultaneous oscillation of fundamental modes, radiation beams converted from second harmonic modes can be separated from the fundamental mode beams by making use of the different radiation directions emitted from the gyrotron window.