目的 探讨人CXC型趋化因子配体16(CXCL16)及其受体CXC型趋化因子受体6(CXCR6)在强直性脊柱炎(AS)发病中的表达及作用机制.方法 通过实时定量聚合酶链反应(Real-time PCR)技术和酶联免疫吸附试验(ELISA)对AS患者外周血及关节液进行CXCL16表达的分析;通过流式细胞术,检测AS患者和对照组外周血淋巴细胞和单核细胞中CXCR6和CXCL16的表达量;趋化实验研究不同浓度的CXCL16对AS患者关节液和外周血中淋巴细胞的趋化作用.结果 AS患者受累关节局部滑液中CXCL16蛋白浓度[(357.90±60.44) pg/ml]以及血浆中CXCL16蛋白浓度[(175.10±13.28) pg/ml]与健康对照组[(122.80±21.42) pg/ml]比较均明显升高(P<0.01);在外周血淋巴细胞中AS患者CXCR6的表达量为(48.63±2.60)%,明显高于健康对照组[(37.70±2.37)%,P<0.01];CXCL16对AS患者关节积液中的淋巴细胞趋化能力明显高于外周血.结论 趋化因子CXCL16及其受体CXCR6在AS患者关节积液和外周血中的表达明显高于对照组,可为AS的免疫治疗提供新的治疗靶位和方法.
Objective To investigate the expression of human CXC chemokine ligand 16 (CXCL16) and its CXC chemokine receptor 6 (CXCR6) in ankylosing spondylitis.Methods The mRNA levels of CXCL16 and CXCR6 in the synovial joints fluid and the peripheral blood of AS patients were respectively measured by Real-time PCR and the protein concentration of CXCL16 in serum was tested by enzyme linked immunosorbent assay (ELISA).The expression of CXCR6 and CXCL16 on lymphocytes and monocytes in the peripheral blood were detected by flow cytometry.A chemotaxis experiment was conducted to test a chemo-attractive activity of different concentration of CXCL16 on mediating lymphocytes trafficking in the synovial joints fluid and the peripheral blood of AS patients.Results CXCL16 protein concentrations expression were significantly increased in the peripheral blood (175.10 ± 13.28) pg/ml and synovial joints fluid (357.90 ± 60.44) pg/ml of the AS patients compared with healthy controls (122.80 ± 21.42) pg/ml,P < 0.01;The frequency of CXCR6 in the peripheral blood lymphocytes of AS patients was higher than in the healthy controls (48.63 ± 2.60) % verse (37.70 ± 2.37) %;P < 0.01).The chemotaxis activity of CXCL16 in synovial joints fluid was significantly higher than that in the peripheral blood lymphocytes of AS patients.Conclusion The gene and protein expression of CXCL16/CXCR6 is significantly higher in the peripheral blood and synovial joints fluid than those in the control group,these data indicating novel immnnotherapeutic and targets of AS.