研究了脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)对 BALB/c 小鼠的毒性作用。结果表明:DON 对BALB/c 小鼠的 LD50为3.3 mg/(kg·bw)。0.6 mg/(kg·bw)和2.4 mg/(kg·bw)DON 组小鼠体重和采食量显著低于对照组和0.15 mg/(kg·bw)DON 组;2.4 mg/(kg·bw)DON 组肝脏、肾脏和脾脏系数显著高于对照组和0.15 mg/(kg·bw)DON 组。病理组织学结果显示,2.4 mg/(kg·bw)DON 组肝脏组织表面有白色坏死灶,肝细胞间质出现红细胞浸润;肾脏明显肿大,肾小管管腔狭窄,肾小球萎缩。与对照组相比,2.4 mg/(kg·bw)DON 组淋巴细胞百分比显著下降;单核细胞百分比、中性粒细胞百分比和红细胞压积显著升高;脾脏 CD4+/CD8+也显著低于对照组和0.15 mg/(kg·bw)DON 组。可见,2.4 mg/(kg·bw)DON 可对 BALB/c 小鼠造成显著的肝脏、肾脏毒性以及免疫抑制作用。
The toxic effect of deoxynivalenol(DON)on BALB/c mice was studied.The results showed that the acute LD50 dosage of DON was 3.3 mg/(kg·bw).The body weight and feed intake of 0.6,2.4 mg/(kg·bw) DON treatment groups were significantly lower than those of the control and 0.15 mg/(kg·bw)DON group.Com-pared with the control and 0.15 mg/(kg·bw)DON group,the liver,spleen and kidney coefficient of 2.4 mg/(kg·bw)DON group were significantly increased.Histopathology results showed that in 2.4 mg/(kg·bw) DON group,there were white necrotic foci on liver tissue surface with erythrocyte infiltration in the cell intersti-tial.The kidney swelled obviously,the renal tubule lumen was narrow and the glomerular atrophied.The lympho-cytes percentage (LY%)significantly decreased,while the percentage of monocytes (MO%),granulocytes (GR%)and hematocrit(HCT)significantly increased.The ratio of CD4 +/CD8 +in spleen of 2.4 mg/(kg·bw) DON group was also significantly decreased compared to the control and 0.15 mg/(kg·bw)DON group.The liver,kidney toxicity of BALB/c mice and immune inhibitory effect were caused in 2.4 mg/(kg·bw)DON group.