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요약정보
一个新的水稻脆秆突变体bc17的鉴定及基因定位 / Identification and gene localization of a novel rice brittle culm mutant bc17
Resource Type
Academic Journal
Authors
姜鸿瑞
;
叶亚峰
;
何丹
;
任艳
;
杨阳
;
谢建
;
程维民
;
陶亮之
;
周利斌
;
吴跃进
;
刘斌美
;
JIANG Hong-Rui
;
YE Ya-Feng
;
HE Dan
;
REN Yan
;
YANG Yang
;
XIE Jian
;
CHENG Wei-Min
;
TAO Liang-Zhi
;
ZHOU Li-Bin
;
WU Yue-Jin
;
LIU Bin-Mei
Source
作物学报 / Acta Agronomica Sinica
. 47(1):71-79
Subject
水稻
脆秆突变体
细胞壁
基因定位
纤维素含量
Language
Chinese
ISSN
0496-3490
Online Access
Scopus
Find it @ DONGA
초록
利用重离子辐照武运粳7号(Wuyunjing 7,wyj7)获得一个脆秆突变体bc17(brittle culm 17),该突变体脆性特征仅在茎秆中表现,叶片正常,并且茎秆脆性在抽穗后开始表现,随着成熟度的增加脆性特征逐渐显著.农艺性状分析表明,该突变体生长发育受到影响,株高显著低于野生型,分蘖数减少以及结实率降低.茎秆和叶片生化成分测定显示,与野生型相比,bc17茎秆和叶片的纤维素含量分别降低22.70%和18.67%,半纤维素含量分别升高45.76%和31.36%.bc17茎秆的抗折力、拉伸力均显著低于野生型,表明茎秆的机械强度发生改变.组织解剖学观察发现,bc17茎秆的厚壁细胞孔隙变大,结构疏松,细胞数目减少.遗传分析表明,bc17的脆秆特征受单隐性核基因控制.利用图位克隆技术将bc17基因精细定位于水稻第7号染色体162 kb区域中,生物信息学分析表明可能是一个新的水稻脆秆基因,为揭示水稻细胞壁合成分子机制的研究提供重要的材料支撑.
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