Core–shell PLA–PVA porous microparticles as carriers for bacteriocin nisin
- Resource Type
- Authors
- Pavlina Holcapkova; Vladimir Sedlarik; Petra Stoplova; Martina Hrabalikova
- Source
- Journal of Microencapsulation. 34:243-249
- Subject
- Vinyl alcohol
Materials science
Scanning electron microscope
Polyesters
Pharmaceutical Science
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Glutarates
chemistry.chemical_compound
Colloid and Surface Chemistry
Polylactic acid
Zeta potential
Organic chemistry
Particle Size
Physical and Theoretical Chemistry
Microparticle
Nisin
Antibacterial agent
Drug Carriers
Organic Chemistry
021001 nanoscience & nanotechnology
Listeria monocytogenes
Anti-Bacterial Agents
0104 chemical sciences
chemistry
Chemical engineering
Polyvinyl Alcohol
Microscopy, Electron, Scanning
0210 nano-technology
Porosity
BET theory
- Language
- ISSN
- 1464-5246
0265-2048
This work is focused on preparation of novel porous type of core-shell-structured microparticles based on polylactide (shell) and poly(vinyl alcohol) cross-linked with glutaric acid (GA) (core) prepared by water-in-oil-in-water solvent evaporation technique. The microparticle systems were used as delivery systems for immobilisation of model antibacterial agent - nisin. The effect of cross-linking and the initial amount of nisin on their morphology was investigated using scanning electron microscopy, BET analysis, zeta potential measurement and Fourier transform infra-red spectroscopy. Encapsulation efficiency and release profile of nisin from the microparticles were studied by high performance liquid chromatography. Antibacterial activity of the prepared systems was tested by dilution and spread plate technique. Results showed the microparticles in the size range of 9-16 μm in diameter with spherical multi-hollow core-shell structure. The presence of cross-linking agent GA influences the release profile of the peptide and has synergistic effect on Listeria monocytogenes growth reduction.