Production and rheological characteristics of the microbial polysaccharide gellan
- Resource Type
- Authors
- Pradiptya Ghosh; A. Gambhir; B. Manna
- Source
- Letters in Applied Microbiology. 23:141-145
- Subject
- chemistry.chemical_classification
Sphingomonas paucimobilis
biology
Microfiltration
Membrane fouling
Ultrafiltration
equipment and supplies
biology.organism_classification
Polysaccharide
Applied Microbiology and Biotechnology
Propanol
Solvent
chemistry.chemical_compound
chemistry
Biochemistry
Fermentation
Food science
- Language
- ISSN
- 0266-8254
B. MANNA, A. GAMBHIR AND P. GHOSH. 1996. Sphingomonas paucimobilis was used for the synthesis of the microbial polysaccharide gellan. In a 60 h fermentation, polysaccharide yield and productivity obtained were 0.45 g gellan per g glucose consumed and 0.21 g I−1 h−1 respectively. The broth showed pseudoplastic behaviour with yield stress. The requirement of the solvent propanol to precipitate gellan from the broth depended on the volume of the broth rather than on gellan concentration. The addition of salt to the broth reduced the propanol requirement. Attempts to separate cells from the highly viscous broth by microfiltration were not successful. Ultrafiltration reduced the propanol requirement but appreciable membrane fouling and loss of gellan was observed.